An amplified impedimetric aptasensor combining target-induce DNA Hydrogel formation with pH-stimulated signal amplification for heparanase assay
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چکیده
An amplified impedimetric aptasensor combining target-induce DNA Hydrogel formation with pH-stimulated signal amplification for heparanase assay Zhe-Han Yang, Ying Zhuo, Ruo Yuan*, Ya-Qin Chai* Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China Materials Tris(hydroxymethyl) aminomethane (Tris), acetic acid, sodium nitrate, ammonium persulfate (APS), magnesium nitrate, gold chloride (HAuCl4·4H2O), N,N,N′,N′tetramethylethylenediamine (TEMED), hexane thiol (HT) and acrylamide solution (40%) were purchased from Sigma–Aldrich (St. Louis, MO, USA). All Freeze-dried nucleic acid strands were purchased from Sangon Biotech (Shanghai) Co., Ltd, and the sequences are showed Table S1: Table S1. Oligonucleotide sequences
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